Hotspot

ycf1

SSC · 120281-125773 · 5493 bp

Back to Pseudostellaria

Pi 0.00803

MarkerSeek score 48.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 116
pi: 0.00803
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGCGATATTGAAGGACAGA	AAAATCAAGAAAGGGTTGAAAA	50.0	27.3	57.5	52.9	202 bp	1.0	44.4
ycf1_p2	CCGCGATATTGAAGGACAGA	AAATCAAGAAAGGGTTGAAAAA	50.0	27.3	57.5	52.9	201 bp	1.0	44.4
ycf1_p3	TCCGCGATATTGAAGGACAG	AAATCAAGAAAGGGTTGAAAAA	50.0	27.3	57.5	52.9	202 bp	1.0	44.4
ycf1_p4	CCGCGATATTGAAGGACAGA	AAAAATCAAGAAAGGGTTGAAA	50.0	27.3	57.5	52.9	203 bp	1.0	44.3
ycf1_p5	TCCGCGATATTGAAGGACAG	AAAATCAAGAAAGGGTTGAAAA	50.0	27.3	57.5	52.9	203 bp	1.0	44.3