Hotspot

ndhF-rpl32

SSC · 109173-109894 · 722 bp

Back to Pseudostellaria

Pi 0.0206
MarkerSeek score 62.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
722
variable_sites
36
pi
0.0206
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTGGAATGAAGGATATGATACATGC GCTGCCCAATACCCTTTTT 40.0 47.4 57.9 56.0 839-880 bp 1.0 47.3
ndhF-rpl32_p2 TGGAATGAAGGATATGATACATGCA GCTGCCCAATACCCTTTTT 36.0 47.4 58.2 56.0 838-879 bp 1.0 47.3
ndhF-rpl32_p3 GGAATGAAGGATATGATACATGCA GCTGCCCAATACCCTTTTT 37.5 47.4 56.7 56.0 837-878 bp 1.0 47.3
ndhF-rpl32_p4 TGGAATGAAGGATATGATACATGC GCTGCCCAATACCCTTTTT 37.5 47.4 56.7 56.0 838-879 bp 1.0 47.3
ndhF-rpl32_p5 GGAATGAAGGATATGATACATGCAT GCTGCCCAATACCCTTTTT 36.0 47.4 57.1 56.0 837-878 bp 1.0 47.3