Hotspot

psaJ-rpl33

LSC · 64488-64873 · 386 bp

Back to Pseudostellaria

Pi 0.0185
MarkerSeek score 61.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
386
variable_sites
20
pi
0.0185
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTGGGTCTTTAGCCGGTCTA CGGACATCTTTCCCCTTAGC 50.0 55.0 58.1 58.0 495-523 bp 1.0 86.4
psaJ-rpl33_p2 CGAACTGAACTAAGAGCGCT CGGACATCTTTCCCCTTAGC 50.0 55.0 58.0 58.0 1089-1123 bp 1.0 85.4
psaJ-rpl33_p3 GCGTTCTACCGAACTGAACT CGGACATCTTTCCCCTTAGC 50.0 55.0 57.9 58.0 1098-1132 bp 1.0 84.9
psaJ-rpl33_p4 CTGCGCTACATCCCTTTCAT CGGACATCTTTCCCCTTAGC 50.0 55.0 58.0 58.0 841-869 bp 1.0 84.6
psaJ-rpl33_p5 CGAACTGAACTAAGAGCGCT GGAAACGCCTCTTGATTCCT 50.0 50.0 58.0 57.9 1159-1193 bp 1.0 84.4