Hotspot

psaA-ycf3

LSC · 40337-41321 · 985 bp

Back to Pseudostellaria

Pi 0.00955

MarkerSeek score 65.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 26
pi: 0.00955
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	1182-1264 bp	1.0	83.6
psaA-ycf3_p2	TGAAAAATGACCGGGTCTGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.1	57.8	1115-1197 bp	1.0	82.8
psaA-ycf3_p3	ACGACGTTTTTACGGGATCC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.3	58.0	1150-1232 bp	1.0	82.2
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	1178-1260 bp	1.0	81.8
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	1180-1262 bp	1.0	81.8