Hotspot

rps16-trnQ-UUG

LSC · 5961-6650 · 690 bp

Back to Pseudostellaria

Pi 0.0199

MarkerSeek score 65.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 39
pi: 0.0199
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	791-835 bp	1.0	85.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	770-814 bp	1.0	85.4
rps16-trnQ-UUG_p3	CCACAACGGATCATGTCCTT	ATAGAAATCGAAGTGGGGCG	50.0	50.0	57.9	57.8	822-866 bp	1.0	84.9
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	781-825 bp	1.0	84.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	ATAGAAATCGAAGTGGGGCG	45.0	50.0	58.1	57.8	801-845 bp	1.0	84.7