Hotspot

trnH-GUG-psbA

LSC · 112-389 · 278 bp

Back to Pseudostellaria

Pi 0.0161

MarkerSeek score 52.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 11
pi: 0.0161
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	386-418 bp	1.0	84.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	400-432 bp	1.0	84.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	399-431 bp	1.0	84.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	401-433 bp	1.0	84.8
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.5	58.0	415-447 bp	1.0	81.9