Hotspot

ycf60

LSC · 33200-34393 · 1194 bp

Back to Pseudostaurastrum

Pi 0.209

MarkerSeek score 80.80

Primer available yes

Label ycf60

Feature Metadata

label_name: ycf60
feature_type: gene
region: LSC
length_bp: 1194
variable_sites: 249
pi: 0.209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf60_p1	TTGATCCAAGGCCTTCATCG	ACTGGAGATCTTGATGCTTGT	50.0	42.9	58.0	57.0	2262 bp	0.5	43.7
ycf60_p2	ATAAAAACTCACGGCTGCCA	ACTGGAGATCTTGATGCTTGT	45.0	42.9	58.1	57.0	1699 bp	0.5	43.3
ycf60_p3	TCTGCCACACGCTTCTAAAA	ACTGGAGATCTTGATGCTTGT	45.0	42.9	57.8	57.0	2060 bp	0.5	42.0
ycf60_p4	AACGTGTTGGCTCAATCCAA	ACTGGAGATCTTGATGCTTGT	45.0	42.9	58.3	57.0	2085 bp	0.5	41.5
ycf60_p5	ACGTGTTGGCTCAATCCAAA	ACTGGAGATCTTGATGCTTGT	45.0	42.9	58.3	57.0	2084 bp	0.5	41.5