Hotspot

ycf19

LSC · 78191-78820 · 630 bp

Back to Pseudostaurastrum

Pi 0.248

MarkerSeek score 78.90

Primer available yes

Label ycf19

Feature Metadata

label_name: ycf19
feature_type: gene
region: LSC
length_bp: 630
variable_sites: 156
pi: 0.248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf19_p1	AACTGTAGATGATGGCTGCG	AATTGACGTGCGGCTAAGAT	50.0	45.0	58.1	58.0	1511 bp	0.5	59.4
ycf19_p2	AACTGTAGATGATGGCTGCG	CCGTCGTTCGCCCTTTTATA	50.0	50.0	58.1	58.1	974 bp	0.5	59.0
ycf19_p3	GACCAAAACCATTGCCCAAC	AATTGACGTGCGGCTAAGAT	50.0	45.0	58.1	58.0	1646 bp	0.5	58.8
ycf19_p4	CCCAAACCACCATGGCTAAT	AATTGACGTGCGGCTAAGAT	50.0	45.0	58.1	58.0	1680 bp	0.5	58.7
ycf19_p5	GACCAAAACCATTGCCCAAC	CCGTCGTTCGCCCTTTTATA	50.0	50.0	58.1	58.1	1109 bp	0.5	58.4