Hotspot

rps5

SSC · 14418-15047 · 630 bp

Back to Pseudostaurastrum

Pi 0.152

MarkerSeek score 80.80

Primer available yes

Label rps5

Feature Metadata

label_name: rps5
feature_type: gene
region: SSC
length_bp: 630
variable_sites: 94
pi: 0.152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps5_p1	TCTTCTTCTGCATCTTGCCG	AGATTCGTGCAATTCGTCCA	50.0	45.0	58.3	57.9	1383 bp	0.5	57.1
rps5_p2	TCTTCTTCTGCATCTTGCCG	CAATTCGTCCACCTGAACCA	50.0	50.0	58.3	58.1	1374 bp	0.5	57.0
rps5_p3	TCTTCTTCTGCATCTTGCCG	GATTCGTGCAATTCGTCCAC	50.0	50.0	58.3	57.8	1382 bp	0.5	56.6
rps5_p4	TCTTCTTCTGCATCTTGCCG	TCGTCCACCTGAACCATACA	50.0	50.0	58.3	58.4	1370 bp	0.5	54.9
rps5_p5	TCTTCTTCTGCATCTTGCCG	CGTCCACCTGAACCATACAA	50.0	50.0	58.3	57.5	1369 bp	0.5	54.1