Hotspot

rpoC2

LSC · 56953-61026 · 4074 bp

Back to Pseudostaurastrum

Pi 0.186

MarkerSeek score 74.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4074
variable_sites: 758
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATGACCAGAATCGGCTGTTC	GCTTTCAACGCGGATTTTGA	50.0	45.0	58.0	58.0	1349 bp	0.5	59.4
rpoC2_p2	ATGACCAGAATCGGCTGTTC	AGCTTTCAACGCGGATTTTG	50.0	45.0	58.0	57.9	1350 bp	0.5	59.4
rpoC2_p3	ATGACCAGAATCGGCTGTTC	CAGCTTTCAACGCGGATTTT	50.0	45.0	58.0	57.9	1351 bp	0.5	59.4
rpoC2_p4	GCGTAACAAAACCCAGCTTC	GCTTTCAACGCGGATTTTGA	50.0	45.0	58.0	58.0	895 bp	0.5	59.3
rpoC2_p5	CATTAGTCCCCGCATTCCAA	GCTTTCAACGCGGATTTTGA	50.0	45.0	57.9	58.0	1205 bp	0.5	59.2