Hotspot

groEL

LSC · 80424-82010 · 1587 bp

Back to Pseudostaurastrum

Pi 0.216

MarkerSeek score 82.50

Primer available yes

Label groEL

Feature Metadata

label_name: groEL
feature_type: gene
region: LSC
length_bp: 1587
variable_sites: 329
pi: 0.216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL_p1	CCCTCCTAGCTCGTCCTTAA	GGTCGTGTTCCATTATGGCT	55.0	50.0	57.9	58.0	2115-2161 bp	1.0	89.5
groEL_p2	CCCTCCTAGCTCGTCCTTAA	TATGACAAGTGCTGGTCGTG	55.0	50.0	57.9	57.9	2128-2174 bp	1.0	89.2
groEL_p3	CCCTCCTAGCTCGTCCTTAA	AGCGGTTCCAACTGTTTTCT	55.0	45.0	57.9	57.9	2404-2439 bp	1.0	89.1
groEL_p4	CCCTCCTAGCTCGTCCTTAA	TGGATTAGCGGTTCCAACTG	55.0	50.0	57.9	57.9	2445 bp	0.5	58.6
groEL_p5	CCCTCCTAGCTCGTCCTTAA	GGATTAGCGGTTCCAACTGT	55.0	50.0	57.9	57.9	2444 bp	0.5	58.6