Hotspot

clpN

SSC · 107151-107672 · 522 bp

Back to Pseudostaurastrum

Pi 0.289

MarkerSeek score 77.20

Primer available yes

Label clpN

Feature Metadata

label_name: clpN
feature_type: gene
region: SSC
length_bp: 522
variable_sites: 151
pi: 0.289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpN_p1	TAGCTGGAAAAAGTGCGGTT	GGCGAAACTCTTCTAGGCAA	45.0	50.0	58.0	57.9	1590 bp	0.5	59.0
clpN_p2	TAGCTGGAAAAAGTGCGGTT	TTGGCGAAACTCTTCTAGGC	45.0	50.0	58.0	57.9	1592 bp	0.5	59.0
clpN_p3	TTAGCTGGAAAAAGTGCGGT	GGCGAAACTCTTCTAGGCAA	45.0	50.0	58.0	57.9	1591 bp	0.5	59.0
clpN_p4	TTAGCTGGAAAAAGTGCGGT	TTGGCGAAACTCTTCTAGGC	45.0	50.0	58.0	57.9	1593 bp	0.5	59.0
clpN_p5	TAGCTGGAAAAAGTGCGGTT	CACAACCCTCAGCTAGCAAA	45.0	50.0	58.0	58.1	1785 bp	0.5	59.0