Hotspot

chlI

SSC · 109124-110308 · 1185 bp

Back to Pseudostaurastrum

Pi 0.15

MarkerSeek score 79.20

Primer available yes

Label chlI

Feature Metadata

label_name: chlI
feature_type: gene
region: SSC
length_bp: 1185
variable_sites: 178
pi: 0.15
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI_p1	TTGAATGATTGGCAACGTGC	TCCTGGGTGGTGAAACAATG	45.0	50.0	57.9	58.0	2263 bp	0.5	59.4
chlI_p2	TTGAATGATTGGCAACGTGC	TATGTGTCACCACGCTCATG	45.0	50.0	57.9	58.0	2057 bp	0.5	59.4
chlI_p3	TTGAATGATTGGCAACGTGC	CGCACTTCTTCCTAGGGTTC	45.0	55.0	57.9	58.0	2026 bp	0.5	59.4
chlI_p4	ACTTCAGGGATGGCAAAGTG	TCCTGGGTGGTGAAACAATG	50.0	50.0	58.1	58.0	2330 bp	0.5	59.3
chlI_p5	ACTTCAGGGATGGCAAAGTG	TATGTGTCACCACGCTCATG	50.0	50.0	58.1	58.0	2124 bp	0.5	59.3