Hotspot

atpB

LSC · 68240-69721 · 1482 bp

Back to Pseudostaurastrum

Pi 0.1

MarkerSeek score 81.10

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 148
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GCCGTCAAGAAAAAGAAGCC	ATTGGTATCCAACGCCCTTC	50.0	50.0	57.9	57.9	2168 bp	0.5	59.2
atpB_p2	GCCGTCAAGAAAAAGAAGCC	GGTATCCAACGCCCTTCAAT	50.0	50.0	57.9	57.9	2165 bp	0.5	59.2
atpB_p3	GCCGTCAAGAAAAAGAAGCC	CTCCGCTTAAGGTAGGGAGA	50.0	55.0	57.9	57.9	2077 bp	0.5	59.0
atpB_p4	GCCGTCAAGAAAAAGAAGCC	TCTCCGCTTAAGGTAGGGAG	50.0	55.0	57.9	57.9	2078 bp	0.5	59.0
atpB_p5	GCCGTCAAGAAAAAGAAGCC	GGAGAACTGCTTGCTGAACT	50.0	50.0	57.9	58.1	2062 bp	0.5	58.7