Hotspot

trnF(gaa)-trnC(gca)

SSC · 113544-113662 · 119 bp

Back to Pseudostaurastrum

Pi 0.294

MarkerSeek score 79.70

Primer available yes

Label trnF(gaa)-trnC(gca)

Feature Metadata

label_name: trnF(gaa)-trnC(gca)
feature_type: igs
region: SSC
length_bp: 119
variable_sites: 32
pi: 0.294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(gaa)-trnC(gca)_p1	GCGACACATGGATCTTCAGT	TTTGCAATCCCCTGCCTTAC	50.0	50.0	58.0	58.4	199-206 bp	1.0	88.3
trnF(gaa)-trnC(gca)_p2	GCGACACATGGATCTTCAGT	ATTTGCAATCCCCTGCCTTA	50.0	45.0	58.0	57.5	200-207 bp	1.0	87.4
trnF(gaa)-trnC(gca)_p3	GCGACACATGGATCTTCAGT	TTACCACTTGGCTATACCGC	50.0	50.0	58.0	57.4	183-190 bp	1.0	87.0
trnF(gaa)-trnC(gca)_p4	GCGACACATGGATCTTCAGT	AATCCCCTGCCTTACCACTT	50.0	50.0	58.0	58.6	194-201 bp	1.0	86.9
trnF(gaa)-trnC(gca)_p5	CCAGAGCCAGACTTGAACTG	TTTGCAATCCCCTGCCTTAC	55.0	50.0	58.2	58.4	219-226 bp	1.0	86.5