Hotspot

trnI(gat)-rns

IRa · 92666-92733 · 68 bp

Back to Pseudostaurastrum

Pi 0.0714

MarkerSeek score 81.20

Primer available yes

Label trnI(gat)-rns

Feature Metadata

label_name: trnI(gat)-rns
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 4
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(gat)-rns_p1	TACCAGCTGAGCTATACCCC	GACCGAGGTGAAGTCGTAAC	55.0	55.0	58.0	58.0	215-227 bp	1.0	85.2
trnI(gat)-rns_p2	TGGACTTGAACCAGAGACCT	GACCGAGGTGAAGTCGTAAC	50.0	55.0	57.9	58.0	181-193 bp	1.0	84.7
trnI(gat)-rns_p3	GCTGGGCTATTCTGGACTTG	GACCGAGGTGAAGTCGTAAC	55.0	55.0	58.3	58.0	193-205 bp	1.0	82.8
trnI(gat)-rns_p4	TACCAGCTGAGCTATACCCC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	58.0	57.6	205-217 bp	1.0	82.6
trnI(gat)-rns_p5	TACCAGCTGAGCTATACCCC	TGACCGAGGTGAAGTCGTAA	55.0	50.0	58.0	58.4	216-228 bp	1.0	82.2