Hotspot

ycf12-rbcS

LSC · 47864-48017 · 154 bp

Back to Pseudostaurastrum

Pi 0.122

MarkerSeek score 80.50

Primer available yes

Label ycf12-rbcS

Feature Metadata

label_name: ycf12-rbcS
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 18
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-rbcS_p1	AGTTGACTAAGAACCTGCAA	TCCTCCTGGAGAAAGATACGT	40.0	47.6	54.5	57.6	202-208 bp	1.0	50.8
ycf12-rbcS_p2	AAGTTGACTAAGAACCTGCA	TCCTCCTGGAGAAAGATACGT	40.0	47.6	54.5	57.6	203-209 bp	1.0	50.7
ycf12-rbcS_p3	AGTTGACTAAGAACCTGCAAA	TCCTCCTGGAGAAAGATACGT	38.1	47.6	55.4	57.6	202-208 bp	1.0	49.8
ycf12-rbcS_p4	AAGTTGACTAAGAACCTGCAA	TCCTCCTGGAGAAAGATACGT	38.1	47.6	55.4	57.6	203-209 bp	1.0	49.7
ycf12-rbcS_p5	ACGCAATAAGTTGACTAAGAACC	TCCTCCTGGAGAAAGATACGT	39.1	47.6	57.1	57.6	210-216 bp	1.0	49.4