Hotspot

petG-trnW-CCA

LSC · 64479-64604 · 126 bp

Back to Pseudosedum

Pi 0.0909

MarkerSeek score 81.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 11
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	AACGTGGTGATCAGTTGGAC	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.1	58.0	169-174 bp	1.0	86.5
petG-trnW-CCA_p2	CGTGGTGATCAGTTGGACTT	GGTTCAAGTCCTACAGAGCG	50.0	55.0	57.8	58.0	167-172 bp	1.0	86.2
petG-trnW-CCA_p3	AACGTGGTGATCAGTTGGAC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	200-205 bp	1.0	85.1
petG-trnW-CCA_p4	AACGTGGTGATCAGTTGGAC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	222-227 bp	1.0	84.6
petG-trnW-CCA_p5	CGTGGTGATCAGTTGGACTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	220-225 bp	1.0	84.3