Hotspot

petA-psbJ

LSC · 61355-62286 · 932 bp

Back to Pseudosedum

Pi 0.0919

MarkerSeek score 82.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 84
pi: 0.0919
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CAGGATTGGGTTCATCCTTGT	50.0	47.6	57.8	57.9	1057-1122 bp	1.0	77.5
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	CAGGATTGGGTTCATCCTTGT	50.0	47.6	58.2	57.9	1072-1137 bp	1.0	77.2
petA-psbJ_p3	TCCATTACGTGTTCAAGGCC	CAGGATTGGGTTCATCCTTGT	50.0	47.6	58.2	57.9	1073-1138 bp	1.0	77.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCAGGATTGGGTTCATCCTTG	50.0	47.6	57.8	57.6	1058-1123 bp	1.0	75.3
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	TCAGGATTGGGTTCATCCTTG	50.0	47.6	58.2	57.6	1073-1138 bp	1.0	75.0