Hotspot

psbM-trnD-GUC

LSC · 29899-30912 · 1014 bp

Back to Pseudosedum

Pi 0.0918

MarkerSeek score 84.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 89
pi: 0.0918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGCAAGAATATTTACTTCCA	TCAATTGGTAAGAGCACCGC	26.1	50.0	52.7	58.6	1090-1101 bp	1.0	49.7
psbM-trnD-GUC_p2	AATGCAAGAATATTTACTTCCAT	TCAATTGGTAAGAGCACCGC	26.1	50.0	52.4	58.6	1089-1100 bp	1.0	49.7
psbM-trnD-GUC_p3	AAATGCAAGAATATTTACTTCCAT	TCAATTGGTAAGAGCACCGC	25.0	50.0	53.3	58.6	1090-1101 bp	1.0	49.7
psbM-trnD-GUC_p4	AAATGCAAGAATATTTACTTCCA	AATTGGTAAGAGCACCGCC	26.1	52.6	52.7	58.1	1088-1099 bp	1.0	49.7
psbM-trnD-GUC_p5	AATGCAAGAATATTTACTTCCAT	AATTGGTAAGAGCACCGCC	26.1	52.6	52.4	58.1	1087-1098 bp	1.0	49.7