Hotspot

atpH-atpI

LSC · 13935-14612 · 678 bp

Back to Pseudosedum

Pi 0.0889

MarkerSeek score 80.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 60
pi: 0.0889
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGCAGCAATAACGGAAG	TAGGAGAATCGATGGAGGGC	50.0	55.0	58.3	58.4	749-772 bp	1.0	81.6
atpH-atpI_p2	AATCCAGCAGCAATAACGGA	TAGGAGAATCGATGGAGGGC	45.0	55.0	57.6	58.4	751-774 bp	1.0	80.3
atpH-atpI_p3	ATCCAGCAGCAATAACGGAA	TAGGAGAATCGATGGAGGGC	45.0	55.0	57.6	58.4	750-773 bp	1.0	80.3
atpH-atpI_p4	TAACGGAAGCAGCAGAAACC	TAGGAGAATCGATGGAGGGC	50.0	55.0	58.5	58.4	738-761 bp	1.0	79.9
atpH-atpI_p5	GCAGCAGAAACCAGTGGATT	TAGGAGAATCGATGGAGGGC	50.0	55.0	58.7	58.4	730-753 bp	1.0	77.9