Hotspot

atpF-atpH

LSC · 13288-13688 · 401 bp

Back to Pseudosedum

Pi 0.109

MarkerSeek score 80.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 41
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ACAGAATCGGTTACGTTTTTCA	ATTTGCAAATCCCTTTGTTT	36.4	30.0	56.6	52.3	422-446 bp	1.0	51.6
atpF-atpH_p2	ACAGAATCGGTTACGTTTTTCAT	ATTTGCAAATCCCTTTGTTT	34.8	30.0	57.1	52.3	422-446 bp	1.0	51.6
atpF-atpH_p3	ACAGAATCGGTTACGTTTTTCA	TTTGCAAATCCCTTTGTTTAA	36.4	28.6	56.6	52.6	421-445 bp	1.0	51.6
atpF-atpH_p4	CAGAATCGGTTACGTTTTTCA	ATTTGCAAATCCCTTTGTTT	38.1	30.0	54.8	52.3	421-445 bp	1.0	51.6
atpF-atpH_p5	ACAGAATCGGTTACGTTTTTCA	CTTTTATTTGCAAATCCCTTTGT	36.4	30.4	56.6	54.6	427-451 bp	1.0	51.5