Hotspot

trnS-GCU-trnG-GCC

LSC · 8666-9296 · 631 bp

Back to Pseudosedum

Pi 0.125

MarkerSeek score 84.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 76
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	680-686 bp	1.0	58.7
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	679-685 bp	1.0	58.7
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	TACCACTAAACTATACCCGC	50.0	45.0	58.1	54.0	698-704 bp	1.0	58.0
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	681-687 bp	1.0	57.6
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	680-686 bp	1.0	57.6