Hotspot

ndhG

SSC · 113390-113920 · 531 bp

Back to Pseudosasa

Pi 0.000942

MarkerSeek score 30.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	GCCCGATAGATCTACCCCTT	45.0	55.0	58.1	58.1	1392-1407 bp	1.0	79.6
ndhG_p2	ACCATGTTTCGGCTTGTGAT	ATGCCCGATAGATCTACCCC	45.0	55.0	58.1	58.1	1394-1409 bp	1.0	79.0
ndhG_p3	ACCATGTTTCGGCTTGTGAT	TGTGAAGTATGTGTTCGCGT	45.0	45.0	58.1	57.9	1414-1429 bp	1.0	78.9
ndhG_p4	ACCATGTTTCGGCTTGTGAT	ATTGTGTTGAGTACTGCCCG	45.0	50.0	58.1	58.2	1287-1302 bp	1.0	78.6
ndhG_p5	GCTCTAACCATGTTTCGGCT	GCCCGATAGATCTACCCCTT	50.0	55.0	58.3	58.1	1398-1413 bp	1.0	78.2