Hotspot

psbT-psbN

LSC · 73266-73313 · 48 bp

Back to Pseudosasa

Pi 0.0521

MarkerSeek score 65.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 48
variable_sites: 5
pi: 0.0521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	116 bp	1.0	83.1
psbT-psbN_p2	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	121 bp	1.0	83.0
psbT-psbN_p3	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	208 bp	1.0	81.7
psbT-psbN_p4	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	203 bp	1.0	81.7
psbT-psbN_p5	TCTTCTTCCGAGAACCACCT	GTCGCCATCTCCATATCTGG	50.0	55.0	58.0	57.6	209 bp	1.0	78.8