Hotspot

rpl16-rps3

LSC · 78108-79275 · 1168 bp

Back to Pseudoroegneria

Pi 0.00171

MarkerSeek score 60.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 5
pi: 0.00171
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CCTCGGCAGGATTTTCCTTT	GGGTAGACTTCCCCTCCAAA	50.0	55.0	58.2	58.3	1376-1380 bp	1.0	76.6
rpl16-rps3_p2	CCTCGGCAGGATTTTCCTTT	TTCCCCTCCAAACAATTCGC	50.0	50.0	58.2	58.7	1368-1372 bp	1.0	73.3
rpl16-rps3_p3	TCGGCAGGATTTTCCTTTCA	GGGTAGACTTCCCCTCCAAA	45.0	55.0	57.4	58.3	1374-1378 bp	1.0	73.3
rpl16-rps3_p4	CCTCGGCAGGATTTTCCTTT	GGTAGACTTCCCCTCCAAAC	50.0	55.0	58.2	57.2	1375-1379 bp	1.0	73.0
rpl16-rps3_p5	CCTCGGCAGGATTTTCCTTT	CTCCAAACAATTCGCGCTAA	50.0	45.0	58.2	57.1	1363-1367 bp	1.0	72.2