Hotspot

rbcL-psaI

LSC · 55846-57489 · 1644 bp

Back to Pseudoroegneria

Pi 0.00377

MarkerSeek score 48.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1644
variable_sites: 14
pi: 0.00377
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1950-1977 bp	1.0	80.9
rbcL-psaI_p2	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	50.0	50.0	58.0	57.9	2136-2163 bp	1.0	80.8
rbcL-psaI_p3	CTTAACTGGGGGATTCACCG	AACTCGCTATACCTGGCTCT	55.0	50.0	58.0	57.9	2501-2528 bp	1.0	80.6
rbcL-psaI_p4	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2438-2465 bp	1.0	80.6
rbcL-psaI_p5	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2439-2466 bp	1.0	80.6