Hotspot

trnC-GCA-rpoB

LSC · 18182-19339 · 1158 bp

Back to Pseudoroegneria

Pi 0.00173

MarkerSeek score 60.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 5
pi: 0.00173
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1223-1227 bp	1.0	79.9
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1230-1234 bp	1.0	78.8
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1238-1242 bp	1.0	77.6
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1237-1241 bp	1.0	77.6
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	50.0	45.0	58.1	57.6	1302-1306 bp	1.0	77.2