Hotspot

trnS-GCU-psbD

LSC · 7650-8624 · 975 bp

Back to Pseudoroegneria

Pi 0.00205

MarkerSeek score 49.10

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 5
pi: 0.00205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1129-1135 bp	1.0	79.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1107-1113 bp	1.0	78.1
trnS-GCU-psbD_p3	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	55.0	55.0	57.8	58.1	1180-1186 bp	1.0	77.6
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1091-1097 bp	1.0	77.6
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	45.0	50.0	58.0	58.3	1122-1128 bp	1.0	77.4