Hotspot

rpoC1

SSC · 142573-148440 · 5868 bp

Back to Pseudopediastrum

Pi 0.414

MarkerSeek score 72.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: SSC
length_bp: 5868
variable_sites: 1924
pi: 0.414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CTCCGCAAAAGAGCAAAAGG	GCGAAAGAAGAAGAACACGC	50.0	50.0	57.9	58.1	1008 bp	0.5	59.1
rpoC1_p2	GTGCTTGTGGAGTACGTTCA	GCGAAAGAAGAAGAACACGC	50.0	50.0	58.1	58.1	745 bp	0.5	58.4
rpoC1_p3	TGTGCTTGTGGAGTACGTTC	GCGAAAGAAGAAGAACACGC	50.0	50.0	58.1	58.1	746 bp	0.5	58.4
rpoC1_p4	CGCAAGCAAAGCTTCCTTTT	GCGAAAGAAGAAGAACACGC	45.0	50.0	58.1	58.1	1306 bp	0.5	58.4
rpoC1_p5	AAAGTTTACTCACTCGGCCG	GCGAAAGAAGAAGAACACGC	50.0	50.0	58.2	58.1	1028 bp	0.5	57.8