Hotspot

psaB

SSC · 127003-129210 · 2208 bp

Back to Pseudopediastrum

Pi 0.469

MarkerSeek score 77.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: SSC
length_bp: 2208
variable_sites: 826
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	ACTCCTTCGCTTTGCAAAGA	GCGGAAGAAGAGTGCAAGTA	45.0	50.0	58.0	57.9	2489 bp	0.5	59.1
psaB_p2	TTGCTTCACTCCTTCGCTTT	GCGGAAGAAGAGTGCAAGTA	45.0	50.0	58.0	57.9	2496 bp	0.5	59.1
psaB_p3	TTTGCTTCACTCCTTCGCTT	GCGGAAGAAGAGTGCAAGTA	45.0	50.0	58.0	57.9	2497 bp	0.5	59.1
psaB_p4	TTTTGCTTCACTCCTTCGCT	GCGGAAGAAGAGTGCAAGTA	45.0	50.0	58.0	57.9	2498 bp	0.5	59.1
psaB_p5	CACTCCTTCGCTTTGCAAAG	GCGGAAGAAGAGTGCAAGTA	50.0	50.0	58.0	57.9	2490 bp	0.5	59.0