Hotspot

petB

SSC · 149108-149755 · 648 bp

Back to Pseudopediastrum

Pi 0.435

MarkerSeek score 83.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: SSC
length_bp: 648
variable_sites: 265
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGTGCTTCGTTTGGAATCCA	CGTCAATTGTAGATGGCCCA	45.0	50.0	58.0	58.0	828 bp	0.5	59.5
petB_p2	ATTCGAACAACTCCAGGACG	CGTCAATTGTAGATGGCCCA	50.0	50.0	57.9	58.0	1488 bp	0.5	59.1
petB_p3	TGTGCTTCGTTTGGAATCCA	GGCCCAGAGCAAAGATGTAA	45.0	50.0	58.0	57.9	814 bp	0.5	58.8
petB_p4	TCCAAGAGTCCAAACGAAGC	CGTCAATTGTAGATGGCCCA	50.0	50.0	58.1	58.0	812 bp	0.5	58.7
petB_p5	CAGCTGCATTTGCTTTGTGA	CGTCAATTGTAGATGGCCCA	45.0	50.0	57.9	58.0	1099 bp	0.5	58.6