Hotspot

cemA

SSC · 119199-120959 · 1761 bp

Back to Pseudopediastrum

Pi 0.0801

MarkerSeek score 92.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: SSC
length_bp: 1761
variable_sites: 125
pi: 0.0801
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 92.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAAGGAAGCGAAGAAGGAGG	GAGCTTCTTCGCTTTGCTTC	55.0	50.0	58.0	57.8	1946 bp	0.5	57.9
cemA_p2	GGAAGCGAAGAAGGAGGAAG	GAGCTTCTTCGCTTTGCTTC	55.0	50.0	58.0	57.8	1943 bp	0.5	57.9
cemA_p3	TACGGAGAGGAGGAAGTTCC	GAGCTTCTTCGCTTTGCTTC	55.0	50.0	57.9	57.8	1969 bp	0.5	57.0
cemA_p4	CCTACGGAGAGGAGGAAGTT	GAGCTTCTTCGCTTTGCTTC	55.0	50.0	57.9	57.8	1971 bp	0.5	56.9
cemA_p5	GGAAGCGAAGAAGGAGGAAG	TTCTTCGCTTTGCTTCCTCC	55.0	50.0	58.0	58.5	1939 bp	0.5	56.1