Hotspot

petB-rps18

SSC · 149756-150450 · 695 bp

Back to Pseudopediastrum

Pi 0.464

MarkerSeek score 83.40

Primer available yes

Label petB-rps18

Feature Metadata

label_name: petB-rps18
feature_type: igs
region: SSC
length_bp: 695
variable_sites: 304
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-rps18_p1	TGTGCTTCGTTTGGAATCCA	CTTCTTCGCTTCTTCGCATG	45.0	50.0	58.0	57.6	2145 bp	0.5	56.3
petB-rps18_p2	AATTGCTTCTGGAACCCCTG	CTTCTTCGCTTCTTCGCATG	50.0	50.0	58.1	57.6	1901 bp	0.5	56.0
petB-rps18_p3	TGTGCTTCGTTTGGAATCCA	ACCGAAGCAGCAAAAGAGAG	45.0	50.0	58.0	58.5	2075 bp	0.5	55.9
petB-rps18_p4	TACTAGCTGACCAACGGTGA	CTTCTTCGCTTCTTCGCATG	50.0	50.0	58.1	57.6	1701 bp	0.5	55.9
petB-rps18_p5	TCCAAGAGTCCAAACGAAGC	CTTCTTCGCTTCTTCGCATG	50.0	50.0	58.1	57.6	2129 bp	0.5	55.6