Hotspot

tufA-trnV(uac)

SSC · 140289-141492 · 1204 bp

Back to Pseudopediastrum

Pi 0.454

MarkerSeek score 83.20

Primer available yes

Label tufA-trnV(uac)

Feature Metadata

label_name: tufA-trnV(uac)
feature_type: igs
region: SSC
length_bp: 1204
variable_sites: 523
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tufA-trnV(uac)_p1	CAGTAGGTGCAGGTGTTGTT	AAGATATAGGCCCAACCGGA	50.0	50.0	58.0	57.9	1321 bp	0.5	58.8
tufA-trnV(uac)_p2	TTACAGGGCGTGGTACAGTA	AAGATATAGGCCCAACCGGA	50.0	50.0	58.1	57.9	1849 bp	0.5	58.5
tufA-trnV(uac)_p3	ACAGTAGCTACAGGACGTGT	AAGATATAGGCCCAACCGGA	50.0	50.0	58.1	57.9	1835 bp	0.5	58.4
tufA-trnV(uac)_p4	CAATTACAGGGCGTGGTACA	AAGATATAGGCCCAACCGGA	50.0	50.0	57.9	57.9	1852 bp	0.5	58.3
tufA-trnV(uac)_p5	CAGTAGGTGCAGGTGTTGTT	GGACTCGAACCTACGAATGG	50.0	55.0	58.0	57.8	1814 bp	0.5	58.1