Hotspot

DR615_pgr004-trnS(gcu)

IRb · 104143-104858 · 716 bp

Back to Pseudopediastrum

Pi 0.355
MarkerSeek score 86.50
Primer available yes
Label DR615_pgr004-trnS(gcu)

Feature Metadata

label_name
DR615_pgr004-trnS(gcu)
feature_type
igs
region
IRb
length_bp
716
variable_sites
207
pi
0.355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DR615_pgr004-trnS(gcu)_p1 GTTGGAAGTCTGGCAGGAAA CGGATTAGCAATCAGTCGCT 50.0 50.0 58.0 58.1 799-971 bp 1.0 90.7
DR615_pgr004-trnS(gcu)_p2 TGGAAGTCTGGCAGGAAAAC CGGATTAGCAATCAGTCGCT 50.0 50.0 58.0 58.1 797-969 bp 1.0 90.7
DR615_pgr004-trnS(gcu)_p3 ACTTGTTGGAAGTCTGGCAG CGGATTAGCAATCAGTCGCT 50.0 50.0 58.0 58.1 803-975 bp 1.0 90.6
DR615_pgr004-trnS(gcu)_p4 GACTTGTTGGAAGTCTGGCA CGGATTAGCAATCAGTCGCT 50.0 50.0 58.0 58.1 804-976 bp 1.0 90.6
DR615_pgr004-trnS(gcu)_p5 AGGAAAACTCAACTAGCGCC CGGATTAGCAATCAGTCGCT 50.0 50.0 58.2 58.1 785-957 bp 1.0 89.3