Hotspot

rps12-psaJ

LSC · 61273-62355 · 1083 bp

Back to Pseudopediastrum

Pi 0.241
MarkerSeek score 84.00
Primer available yes
Label rps12-psaJ

Feature Metadata

label_name
rps12-psaJ
feature_type
igs
region
LSC
length_bp
1083
variable_sites
212
pi
0.241
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-psaJ_p1 TCGAGCTGTTTTAATTAATTGTTGA ATCGTTTCTTCCCAGATCCA 28.0 45.0 56.1 56.2 1157-1251 bp 1.0 52.0
rps12-psaJ_p2 TCGAGCTGTTTTAATTAATTGTTGA TCGTTTCTTCCCAGATCCAT 28.0 45.0 56.1 56.2 1156-1250 bp 1.0 52.0
rps12-psaJ_p3 TCGAGCTGTTTTAATTAATTGTTGA AATCGTTTCTTCCCAGATCCA 28.0 42.9 56.1 57.0 1158-1252 bp 1.0 52.0
rps12-psaJ_p4 TCGAGCTGTTTTAATTAATTGTTGA TCGTTTCTTCCCAGATCCATT 28.0 42.9 56.1 57.0 1156-1250 bp 1.0 52.0
rps12-psaJ_p5 CGAGCTGTTTTAATTAATTGTTGA TCGTTTCTTCCCAGATCCAT 29.2 45.0 54.7 56.2 1155-1249 bp 1.0 52.0