Hotspot

DR615_pgp039

SSC · 154256-154834 · 579 bp

Back to Pseudopediastrum

Pi 0.439

MarkerSeek score 79.80

Primer available yes

Label DR615_pgp039

Feature Metadata

label_name: DR615_pgp039
feature_type: gene
region: SSC
length_bp: 579
variable_sites: 211
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR615_pgp039_p1	TGCTTTTCCTCCTTCACCAC	GGCCATGGACATATTCGTGT	50.0	50.0	58.0	58.0	1511 bp	0.5	59.6
DR615_pgp039_p2	TGCTTTTCCTCCTTCACCAC	TGGCCATGGACATATTCGTG	50.0	50.0	58.0	58.0	1512 bp	0.5	59.6
DR615_pgp039_p3	CACGCTCTGTACACGAAGAA	GGCCATGGACATATTCGTGT	50.0	50.0	58.0	58.0	1494 bp	0.5	59.4
DR615_pgp039_p4	CACGCTCTGTACACGAAGAA	TGGCCATGGACATATTCGTG	50.0	50.0	58.0	58.0	1495 bp	0.5	59.4
DR615_pgp039_p5	CAGTCCATTGTGCTGGAAGT	GGCCATGGACATATTCGTGT	50.0	50.0	58.1	58.0	1663 bp	0.5	59.0