Hotspot

rps16

LSC · 5316-6469 · 1154 bp

Back to Pseudognaphalium

Pi 0.0118

MarkerSeek score 63.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1154
variable_sites: 26
pi: 0.0118
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAGCAAGAAAAGAAGG	ACTGGTTCCAAATTCGAGGA	39.1	45.0	56.9	56.8	1270-1302 bp	1.0	43.1
rps16_p2	ACGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGA	37.5	45.0	58.2	56.8	1270-1302 bp	1.0	43.1
rps16_p3	CGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGA	39.1	45.0	56.6	56.8	1269-1301 bp	1.0	43.1
rps16_p4	ACGAATAGAGCAAGAAAAGAAGG	ACTGGTTCCAAATTCGAGGAA	39.1	42.9	56.9	57.5	1270-1302 bp	1.0	43.1
rps16_p5	ACGAATAGAGCAAGAAAAGAAGGA	ACTGGTTCCAAATTCGAGGAA	37.5	42.9	58.2	57.5	1270-1302 bp	1.0	43.1