Hotspot

rpl16

LSC · 82049-83471 · 1423 bp

Back to Pseudognaphalium

Pi 0.0125

MarkerSeek score 67.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1423
variable_sites: 41
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2201-2250 bp	1.0	84.2
rpl16_p2	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	2124-2173 bp	1.0	84.2
rpl16_p3	TCGACGATTGCTAGCTCCTA	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2231-2280 bp	1.0	84.1
rpl16_p4	GCGTCTTTGATCACAGCAAC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2275-2324 bp	1.0	84.0
rpl16_p5	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2441-2490 bp	1.0	83.8