Hotspot

accD-psaI

LSC · 59618-60330 · 713 bp

Back to Pseudognaphalium

Pi 0.00547

MarkerSeek score 59.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 9
pi: 0.00547
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	TTGCGATTGCCGGAAATACT	40.0	45.0	55.5	58.3	838-865 bp	1.0	62.5
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	40.0	45.0	55.5	58.3	837-864 bp	1.0	62.5
accD-psaI_p3	TGAACTCCACACTTTCTTTCCT	TTGCGATTGCCGGAAATACT	40.9	45.0	57.5	58.3	814-841 bp	1.0	62.3
accD-psaI_p4	AAAAAGCGTTCTGAGTGAGT	AGGCCTACTAAAGGGACCAA	40.0	50.0	55.5	57.6	818-845 bp	1.0	61.8
accD-psaI_p5	AAAAGCGTTCTGAGTGAGTT	AGGCCTACTAAAGGGACCAA	40.0	50.0	55.5	57.6	817-844 bp	1.0	61.8