Hotspot

trnF-GAA-ndhJ

LSC · 48769-49445 · 677 bp

Back to Pseudognaphalium

Pi 0.00793

MarkerSeek score 61.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 11
pi: 0.00793
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATAGCCGGGATAGCTCAGT	TGGATAGGATGGCCTTTACG	50.0	50.0	58.0	56.8	752-823 bp	1.0	75.2
trnF-GAA-ndhJ_p2	AATAGCCGGGATAGCTCAGT	GGATAGGATGGCCTTTACGT	50.0	50.0	58.0	56.8	751-822 bp	1.0	75.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	709-780 bp	1.0	75.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	708-779 bp	1.0	75.0
trnF-GAA-ndhJ_p5	AATAGCCGGGATAGCTCAGT	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.0	57.7	759-830 bp	1.0	74.6