Hotspot

trnG-UCC-trnfM-CAU

LSC · 36566-36847 · 282 bp

Back to Pseudognaphalium

Pi 0.0447

MarkerSeek score 88.00

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 25
pi: 0.0447
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GAACGAAACAAAAAGCGGGT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.8	58.0	396-497 bp	1.0	85.9
trnG-UCC-trnfM-CAU_p2	GAACGAAACAAAAAGCGGGT	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.8	58.1	390-491 bp	1.0	85.5
trnG-UCC-trnfM-CAU_p3	GAACGAAACAAAAAGCGGGT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	57.8	58.0	432-533 bp	1.0	85.4
trnG-UCC-trnfM-CAU_p4	GAACGAAACAAAAAGCGGGT	TCACGGGTTCAAATCCTGTC	45.0	50.0	57.8	57.8	389-490 bp	1.0	84.9
trnG-UCC-trnfM-CAU_p5	GAACGAAACAAAAAGCGGGT	TGAGGTCACGGGTTCAAATC	45.0	50.0	57.8	57.8	394-495 bp	1.0	84.8