Hotspot

psbZ-trnG-UCC

LSC · 36193-36494 · 302 bp

Back to Pseudognaphalium

Pi 0.00993

MarkerSeek score 44.20

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 6
pi: 0.00993
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	ACTTCATTTTGGGCGGGTAG	50.0	50.0	57.8	58.2	493-496 bp	1.0	78.2
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	GACTTCATTTTGGGCGGGTA	50.0	50.0	57.8	58.2	494-497 bp	1.0	78.1
psbZ-trnG-UCC_p3	GCTTCTCCTGATGGTTGGTC	ACTTCATTTTGGGCGGGTAG	55.0	50.0	58.3	58.2	491-494 bp	1.0	77.8
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	GACTTCATTTTGGGCGGGTA	55.0	50.0	58.3	58.2	492-495 bp	1.0	77.7
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	ATGACTTCATTTTGGGCGGG	50.0	50.0	57.8	58.8	496-499 bp	1.0	73.0