Hotspot

trnT-GGU-psbD

LSC · 31559-32891 · 1333 bp

Back to Pseudognaphalium

Pi 0.0227

MarkerSeek score 72.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 64
pi: 0.0227
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1457-1647 bp	0.8	74.2
trnT-GGU-psbD_p2	TAAGCCCTCGGTTCAAATCC	GAAACGGTCCCTACGTAACC	50.0	55.0	57.6	58.0	1455-1645 bp	0.8	73.1
trnT-GGU-psbD_p3	TGAACCAAGCCCTTTCCTTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.5	58.0	1500-1690 bp	0.8	72.5
trnT-GGU-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1465-1655 bp	0.8	72.3
trnT-GGU-psbD_p5	CGTAAGCCCTCGGTTCAAAT	CGAAACGGTCCCTACGTAAC	50.0	55.0	58.3	58.4	1458-1648 bp	0.8	71.2