Hotspot

trnG-UCC-trnT-GGU

LSC · 31013-31495 · 483 bp

Back to Pseudognaphalium

Pi 0.0704
MarkerSeek score 82.40
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
483
variable_sites
69
pi
0.0704
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ATTTGAACCGAGGGCTTACG 38.1 50.0 55.5 58.3 567-693 bp 0.8 50.1
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT AGGGCTTACGCTTTACACTC 38.1 50.0 55.5 57.6 557-683 bp 0.8 49.3
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT GAGGGCTTACGCTTTACACT 38.1 50.0 55.5 57.6 558-684 bp 0.8 49.3
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT GGGCTTACGCTTTACACTCT 38.1 50.0 55.5 57.6 556-682 bp 0.8 49.3
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT GGATTTGAACCGAGGGCTTA 38.1 50.0 55.5 57.6 569-695 bp 0.8 48.9