Hotspot

trnR-UCU-trnG-UCC

LSC · 29979-30236 · 258 bp

Back to Pseudognaphalium

Pi 0.0652

MarkerSeek score 82.20

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 33
pi: 0.0652
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	312-345 bp	1.0	75.3
trnR-UCU-trnG-UCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	315-348 bp	1.0	75.0
trnR-UCU-trnG-UCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	318-351 bp	1.0	74.5
trnR-UCU-trnG-UCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	321-354 bp	1.0	74.2
trnR-UCU-trnG-UCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	316-349 bp	1.0	72.8