Hotspot

petD

LSC · 76440-77529 · 1090 bp

Back to Pseudogalium

Pi 0.00245

MarkerSeek score 35.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1090
variable_sites: 4
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCCCTTCAACCCCCACTATT	ACGTCGTGCTAGTTCACAAT	50.0	45.0	57.9	57.6	1186 bp	1.0	76.2
petD_p2	TTCCCTTCAACCCCCACTAT	ACGTCGTGCTAGTTCACAAT	50.0	45.0	57.9	57.6	1187 bp	1.0	76.2
petD_p3	TTTCCCTTCAACCCCCACTA	ACGTCGTGCTAGTTCACAAT	50.0	45.0	58.2	57.6	1188 bp	1.0	75.5
petD_p4	TCCCTTCAACCCCCACTATT	TACGTCGTGCTAGTTCACAA	50.0	45.0	57.9	56.9	1187 bp	1.0	71.2
petD_p5	TTCCCTTCAACCCCCACTAT	TACGTCGTGCTAGTTCACAA	50.0	45.0	57.9	56.9	1188 bp	1.0	71.2