Hotspot

ndhF-rpl32

SSC · 112209-112946 · 738 bp

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Pi 0.0199
MarkerSeek score 50.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
738
variable_sites
22
pi
0.0199
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CAAAGGTATGATCCACCCGT TGATGCCGAAGTACGCTTTT 50.0 45.0 57.3 58.2 810-870 bp 1.0 74.9
ndhF-rpl32_p2 CAAAGGTATGATCCACCCGT TTGATGCCGAAGTACGCTTT 50.0 45.0 57.3 58.2 811-871 bp 1.0 74.9
ndhF-rpl32_p3 CAAAGGTATGATCCACCCGT TTTGATGCCGAAGTACGCTT 50.0 45.0 57.3 58.2 812-872 bp 1.0 74.9
ndhF-rpl32_p4 CAAAGGTATGATCCACCCGT TTTTGATGCCGAAGTACGCT 50.0 45.0 57.3 58.2 813-873 bp 1.0 74.9
ndhF-rpl32_p5 TCAAAGGTATGATCCACCCG TGATGCCGAAGTACGCTTTT 50.0 45.0 57.0 58.2 811-871 bp 1.0 72.5