Hotspot

ycf3-trnS-GGA

LSC · 45105-45405 · 301 bp

Back to Pseudogalium

Pi 0.00886

MarkerSeek score 58.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 4
pi: 0.00886
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	463-466 bp	1.0	67.4
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	464-467 bp	1.0	67.4
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	484-487 bp	1.0	66.9
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	485-488 bp	1.0	66.9
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	ACGAAAACGGAAAGAGAGGG	45.0	50.0	56.4	57.8	490-493 bp	1.0	66.8